Nutr Cancer.:葡萄籽提取物能够有效杀死癌细胞
2013年12月5日讯 --科罗拉多大学癌症研究中心科学家在实验室中合成葡萄籽提取物的最有效成分B2G2，该物质能够引起前列腺癌细胞的凋亡，而不会对健康细胞造成伤害。相关报道发表在近期的Nutrition and Cancer杂志上。该文章的通讯作者科罗拉多大学癌症中心研究员Alpna Tyagi博士称，我们之前发现葡萄籽提取物（grape seed extract，GSE）具有抗癌的效果，但是之前我们并不知道该提取物的有效成分。
GSE是多酚类的复合物，组分非常复杂。科学家花费了十多年的时间研究GSE中的抗癌成分，Tyagi博士称，直到现在，我们在实验室合成了B2G2，我们终于找到了GSE中的有效成分。
Tyagi博士称，尽管我们发现B2G2的作用，但是从GSE中提取B2G2是一个非常耗时且昂贵的过程。由于B2G2分离的难度很大，所以科学家揭示其功能的进度缓慢。于是Tyagi博士决定采用实验室合成的方法合成B2G2。合成方法产出量高且不昂贵。科学家进一步研究合成的B2G2的抗癌效果。发现B2G2具有与GSE相同的抗癌能力。
Tyagi博士称，我们还不知道B2G2的作用机制，我们希望进一步的优化合成方法产出更多的B2G2，并研究B2G2的诱发癌细胞凋亡的机制。
doi:10.1080/01635581.2013.785003
Differential Effect of Grape Seed Extract against Human Non-small-Cell Lung Cancer Cells: The Role of Reactive Oxygen Species and Apoptosis Induction
Alpna Tyagi, Komal Raina, Subhash Gangar, Manjinder Kaur, Rajesh Agarwal & Chapla Agarwal
The present study examines grape seed extract (GSE) efficacy against a series of non-small-cell lung cancer (NSCLC) cell lines that differ in their Kras and p53 status to establish GSE potential as a cytotoxic agent against a wide range of lung cancer cells. GSE suppressed growth and induced apoptotic death in NSCLC cells irrespective of their k-Ras status, with more sensitivity toward H460 and H322 (wt k-Ras) than A549 and H1299 cells (mutated k-Ras). Mechanistic studies in A549 and H460 cells, selected, based on comparative efficacy of GSE at higher and lower doses, respectively, showed that apoptotic death involves cytochrome c release associated caspases 9 and 3 activation, and poly (ADP-ribosyl) polymerase cleavage, strong phosphorylation of ERK1/2 and JNK1/2, downregulation of cell survival proteins, and upregulated proapoptotic Bak expression. Importantly, GSE treatment caused a strong superoxide radical-associated oxidative stress, significantly decreased intracellular reduced glutathione levels, suggesting, for the first time, the involvement of GSE-caused oxidative stress in its apoptotic inducing activity in these cells. Because GSE is a widely-consumed dietary agent with no known untoward effects, our results support future studies to establish GSE efficacy and usefulness against NSCLC control.
不靠谱。体外能杀死癌细胞的物质太多了。 没那么神奇，保健品而已。
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